Oxford Nanopore long reads obtained from sequencing the DNA of an Ecuadorian brown-headed spider monkey (Ateles fusciceps fusciceps), were used to assemble and annotate the whole genome of this species. ONT long reads were filtered and trimmed in Nanofilt and Porechop. Sequencing statistics were visualized in Nanoplot. The reads were later processed to generate a genome assembly. Two different assemblers, Flye and Smartdenovo, were used on the raw reads to produce draft genomes. The resulting assemblies were polished in Medaka and analyzed for genome completeness and quality in Quast and BUSCO. The best resulting assembly was later annotated in Maker in 3 consecutive rounds using the ab initio gene predictor SNAP.