This custom minimally morphologically destructive DNA extraction protocol was developed for malaria transmitting Anopheles mosquitoes as a cheap and fast alternative to kit-based approaches. DNA extracted using this method can be used unpurified but diluted for singleplex or multiplex PCR. It can also go unpurified straight into Covaris shearing followed by Illumina library preparation or, if quantification or quality is uncertain, it can be purified and used for whole genome sequencing. It is important to note that the times, temperatures, and volumes suggested here were primarily adapted for use with Anopheles mosquitoes, but these are quite flexible and can be adapted for different Diptera species (such as a 5-fold increase in volume for larger species like Glossina) and probably beyond.