A | B | |
total RNA (1 pg-1ug) | max vol 4ul | |
Water | fill up to 4ul | |
OligodT | 1ul | |
final volume =5ul |
A | B | |
RT buffer (5X) | 4ul | |
MgCl2 (25 mM) | 3ul | |
dNTP Mix (10 mM) | 1 ul | |
Rnasine* | 0.5 ul | |
Rtase* | 1 ul | |
H2O | 5.5ul | |
final volume= 15 ul |
A | B | |
hybridization | 5 min at 25°C | |
elongation | 60 min at 42°C | |
inactivation of the enzyme | 15 min at 70°C |