Nov 05, 2023
Sleep-Wake Recording in Dan lab
- 1University of California, Berkeley
Protocol Citation: Xiaolin Huang 2023. Sleep-Wake Recording in Dan lab. protocols.io https://dx.doi.org/10.17504/protocols.io.x54v9pkd4g3e/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 27, 2023
Last Modified: November 05, 2023
Protocol Integer ID: 89994
Funders Acknowledgement:
ASAP
Grant ID: 020551
Abstract
Surgery and Setups for Recording 24-Hour Natural Sleep/Wake Cycles in Mice.
Surgery
Surgery
Mice were anesthetized with 1.5% isoflurane and placed in a stereotaxic frame.
Body temperature was maintained using a heating pad.
The skin was incised to expose the skull after asepsis and connective tissue was
removed.
To implant electroencephalogram (EEG) and electromyogram (EMG) recording
electrodes, two stainless steel screws were inserted into the skull 2.5 mm from
the midline and 3 mm posterior to the bregma, and two EMG electrodes were also
inserted into the neck muscles.
A reference screw for grounding was placed on top of the left cerebellum.
Insulated leads from the EEG and EMG electrodes were soldered to a pin header, which was
secured to the skull using dental cement.
Recording
Recording
After a minimum of 7 days of undisturbed post-surgery recovery, behavioral
experiments were carried out in home cages placed in sound-attenuating boxes.
EEG and EMG electrodes were connected to flexible recording cables via a
mini-connector.
Recordings started after 1-2 days of habituation and continued for 3-4 days on a 12-h
dark/12-h light cycle.
Mouse behavior was recorded using a video camera at 10 frames per second (fps), along
with EEG and EMG recordings.
The signals were recorded with a TDT RZ10x/PZ5-32 or LR10-SK1 system.