- Transfer 900 µL to 2 mL Eppendorf tubes with pre-aliquoted 600 µL 100% isopropanol. Use pipettes with tips removed.
- Invert gently 50 times!
- Pellet DNA at 13,000 RPM for 2 minutes.
- Discard isopropanol supernatant.
- Wash DNA with 1 mL 70% ethanol.
- Centrifuge at 13,000 RPM for 2 minutes.
- Pour off as much ethanol as possible. Be careful, the pellet may be loose!
- Air dry pellet for at least 15 minutes. This can be done in the safety cabinet, by placing the opening of the tubes over the air flow beneath the glass barrier.
- Rehydrate DNA in ~25 µL (depending on volume of DNA, can be increased) Elution buffer (Qiagen) or TE buffer. Use pipettes with tips removed to suspend DNA by pipetting up and down.