Protocol Citation: Ester Kalef-Ezra, Amy Bowes, Christos Proukakis 2023. Single-cell Whole Genome Amplification (scWGA) of human frozen post-mortem brain samples isolated by Laser Capture Microdissection (LCM). protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvorjw7v4o/v1
Manuscript citation:
REFERENCES:
Part of this protocol was adapted from the publication:
Keinath MC, Timoshevskiy VA, Timoshevskaya NY, Tsonis PA, Voss SR, Smith JJ. Initial characterization of the large genome of the salamander Ambystoma mexicanum using shotgun and laser capture chromosome sequencing. Sci Rep. 2015 Nov 10;5:16413. doi: 10.1038/srep16413. PMID: 26553646; PMCID: PMC4639759
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
Grant ID: 000430
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Abstract
This protocol uses Laser Capture Microdissection (LCM) technology on human post-mortem brain tissue slides with rapid Giemsa staining to isolate single nuclei for Single-cell Whole Genome Amplification (scWGA) in order to do low coverage (<1x) single-cell whole genome sequencing to detect mega-base somatic Copy Number Variations (CNVs).