A | B | C | |
Item | Vendor | Catalog # | |
DMEM/F12 | Thermo Fisher | 11320082 | |
Fetal Bovine Serum (FBS) | Corning | 35-011-CV | |
Knockout Serum Replacement | Thermo Fisher | 10828-028 | |
L-Glutamine | Sigma | G8540 | |
Penicillin & Streptomycin (100X) | Thermo Fisher | 15140163 | |
MEM Non-Essential Amino Acids (100X) | Thermo Fisher | 11140050 | |
Heat Stable Recombinant Human FGF2 | Thermo Fisher | PHG0360 | |
Y-27632 | Chemdea | CD0141 | |
2-Mercaptoethanol | Sigma | M3148 |
A | B | |
DMEM/F12 | 385 ml | |
Fetal Bovine Serum (FBS) | 75 ml | |
Knockout Serum Replacement | 25 ml | |
L-Glutamine (100X) | 5 ml | |
Penicillin & Streptomycin (100X) | 5 ml | |
MEM Non-Essential Amino Acids (100X) | 5 ml | |
2-Mercaptoethanol (10,000X) | 50 µl | |
Heat Stable Recombinant Human FGF2 (25 µg/ml)* | 80 µl |
A | B | |
L-Glutamine, powder | 14.6 g | |
MilliQ H2O | 500 ml |
A | B | |
2-Mercaptoethanol | 0.78 ml | |
MilliQ H2O | 9.22 ml |
A | B | |
Heat Stable Recombinant Human FGF2 | 500 µg | |
0.1% BSA | 20 ml |
A | B | |
hPSCs medium | 500 ml | |
Y-27632 (1,000X) | 500 µl |
A | B | |
Y-27632 | 5 mg | |
DMSO | 1.56 ml |