Jul 06, 2022

Public workspaceSingle cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron

DOI
dx.doi.org/10.17504/protocols.io.261generdg47/v1
  • 1Columbia University Irving Medical Center
  • SPARC
    Tech. support email: info@neuinfo.org
Marlena S Pela
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DOI: dx.doi.org/10.17504/protocols.io.261generdg47/v1
Collection Citation: Seoeun Lee, Daniele Neri, Lori Zeltser 2022. Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron. protocols.io https://dx.doi.org/10.17504/protocols.io.261generdg47/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it’s working
Created: April 12, 2022
Last Modified: July 06, 2022
Collection Integer ID: 60678
Abstract
A collection of protocols to sequence and analyze single neurons from the stellate ganglia of mice after injection of Cholera Toxin B (CTB) in the interscapular brown adipose fat.

Step 1: Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB)

Step 2: Ganglia dissociation and single-cell sorting

Step 3: Single-cell sequencing and analysis
Files
Protocol
Icon representing the file Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB)
Name
Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB)
Version 1
,
Seoeun Lee
Protocol
Icon representing the file Ganglia dissociation and single-cell sorting
Name
Ganglia dissociation and single-cell sorting
Version 1
,
Daniele Neri
Protocol
Icon representing the file Single-cell sequencing and analysis
Name
Single-cell sequencing and analysis
Version 1
,
Daniele Neri