Jul 06, 2022
Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron
- 1Columbia University Irving Medical Center
Collection Citation: Seoeun Lee, Daniele Neri, Lori Zeltser 2022. Single cell RNA sequencing of retrogradely labeled mouse stellate ganglion neuron. protocols.io https://dx.doi.org/10.17504/protocols.io.261generdg47/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it’s working
Created: April 12, 2022
Last Modified: July 06, 2022
Collection Integer ID: 60678
Abstract
A collection of protocols to sequence and analyze single neurons from the stellate ganglia of mice after injection of Cholera Toxin B (CTB) in the interscapular brown adipose fat.
Step 1: Retrograde labeling of brown adipose tissue (BAT)-projecting sympathetic neurons with cholera toxin B (CTB)
Step 2: Ganglia dissociation and single-cell sorting
Step 3: Single-cell sequencing and analysis
