Jan 10, 2024
Single action sequence reinforcement
- Jonathan Tang1,2,3
- 1Department of Neuroscience, Zuckerman Mind Brain Behavior Institute, Columbia University, New York, NY 10027, USA;
- 2Seattle Children’s Research Institute, Center for Integrative Brain Research, Seattle, WA, USA;
- 3University of Washington School of Medicine, Department of Pediatrics, Seattle, WA, USA
Protocol Citation: Jonathan Tang 2024. Single action sequence reinforcement . protocols.io https://dx.doi.org/10.17504/protocols.io.4r3l221k4l1y/v1
Manuscript citation:
Tang, J.C.Y., Paixao, V., Carvalho, F. et al. Dynamic behaviour restructuring mediates dopamine-dependent credit assignment. Nature (2023). https://doi.org/10.1038/s41586-023-06941-5
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 21, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 92643
Keywords: ASAPCRN, mouse, behavior, brain
Funders Acknowledgement:
Life Sciences Research Fellowship
NINDS K99/R00 Award
Grant ID: 1K99NS112575
NIH
Grant ID: 5U19NS104649
Aligning Science Across Parkinson’s (ASAP)
Grant ID: ASAP-020551
Abstract
Single action sequence reinforcement protocol for mouse studies from Tang et al 2023.
Guidelines
Individual mice were subjected to a single session of protocol each day, with sessions following each other on consecutive days.
Materials
The white open field box is made of 410 x 400 mm white matte acrylic walls and a 410 x 400 mm white matte acrylic base. Note: A white open field was used instead of a grey open field to minimize habituation effects, which would lead to reduced initial spontaneous behavior during closed loop protocol.
Session 1 + Baseline
Session 1 + Baseline
Mice were placed in a white open field box for closed loop reinforcement protocol.
To acquire baseline behavior, individual mice were allowed to behave freely inside the box for 30 minutes on the first action A reinforcement session.
After initial behavior acquisition, closed loop reinforcement was initated by blue laser stimulation of VTA DA neurons were made available for 60 minutes.
Sessions 2 & 3
Sessions 2 & 3
90 minutes of closed loop reinforcement were made available for individual mice during sessions.
An extinction protocol was carried out comprising of 20-minute maintenance of reinforced behavior with laser availability, followed by 60 minutes of extinction of reinforced behavior without laser availability, followed by 20-minute re-acquisition of reinforced behavior with laser availability.
Selection for Action B 1 step
To select for action B, a repeat of the protocol described above for action A was performed starting on the day following extinction protocol of action A.
Contingency Degradation Protocol
Contingency Degradation Protocol
Upon completion of the reinforcement and extinction protocols for action B, a contingency degradation protocol was performed comprising of 20-minute maintenance of action B with laser availability, followed by 60 minutes of contingency degradation of reinforced behavior by triggering laser randomly, followed by 40-minute re-acquisition of reinforced behavior with laser availability for action B performance.