SH-SY5Y neuroblastoma cells were cultured in a 1:1 mixture of Ham’s F-12 and DMEM growth medium supplemented with 10% FBS, non-essential amino acids (NEAA: 0.1 mM of: glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline and L-serine) and penicillin/streptomycin antibiotic cocktail (50ng/ml) at 37°C and 5% CO2. Cells were passaged at around 80% confluency using trypsin digestion. Culture medium was removed from cells, centrifuged at 1000 RPM for 5 minutes to remove floating cells and stored at 80°C for analysis. Cells were washed once in PBS and trypsin was added (0.25% trypsin in Versene). Following an incubation of 2 minutes, the plates were tapped to detach cells. The trypsin was neutralised in 9 volumes of normal growth medium. For continued culturing, a fraction of cells was transferred to a falcon tube and pelleted for 10 minutes at 1000 RPM (200 x g). Pellets were resuspended in the appropriate volume of normal medium and split into new plates. If required, cells were counted prior to seeding using the C-Chip NanoEnTek DHC-N01 counter. Cell line stocks were generated by resuspending cell pellets in 1 mL of freezing medium (10% DMSO in FBS).