Oct 17, 2023
Version 1
Set Shifting ASAP Operant Behavior_LernerLab V.1
This protocol is a draft, published without a DOI.
- 1Northwestern University
Protocol Citation: Jillian Seiler 2023. Set Shifting ASAP Operant Behavior_LernerLab. protocols.io https://protocols.io/view/set-shifting-asap-operant-behavior-lernerlab-c3hcyj2w
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 16, 2023
Last Modified: October 17, 2023
Protocol Integer ID: 89348
Abstract
Protocol for set shifting task used by the Lerner Lab for ASAP work
Materials
Med Associates operant chambers
Magazine Training
Magazine Training
Food restrict animals overnight prior to beginning magazine training. For the remainder of training animals should be restricted to 85% of their free feeding weight.
Connect 20 mL syringe with >10 mLs of 1:3 diluted sweetened condensed milk. Add ~15 inches of tubing with tubing adapter to syringe. Load in pump, attach to reward port. Prime pump.
Place animals in operant box with dummy patch cords attached and allow ~5 minutes to habituate
Load JN_ASAP_A_MagTraining_30min into MED-PC and start program. The script for the program is attached as a comment. The overview is as follows:
House light, fan, corner lights, and cue lights above the left and right nosepoke ports are turned on.
On average, every 60 seconds a noncontigent reward is delivered
The animal must consume the reward (or make a port entry) in order for the next reward to be delivered
After 30 minutes, the program ends
This program occurs once, regardless of performance
After animal has finished, record weight, return to homecage and clean operant chamber tray with 70% ethanol
Flush reward port and tubing with ethanol and hang to dry
Store any remaining sweetened condensed milk in refrigerator
FR1 Both
FR1 Both
Repeat steps 2+3 go to step #2
Load JN_ASAP_B_FR1_BOTH and start program. The script for the program is attached as a comment. The overview is as follows:
Fan and Near-Infrared Lights are turned on