A | B | |
Master mix | 1x | |
H2O | 13.3 | |
5x Colorless Reaction Buffer (Promega, #M3005) | 4 | |
10mM dNTPs (ThermoFisher, #R0182) | 0.4 | |
Fw primer (CMV FW) | 0.6 | |
Rv primer (BGH RV) | 0.6 | |
GoTaq Polymerase (Promega, #M3005) | 0.1 | |
Σ | 19 µl |
A | B | |
1x sample | ||
H2O | 5.3 µl | |
5x Terminator Sequencing buffer | 3.3 µl | |
BigDye v3.1 (ThermoFisher, #4337455) | 1.4 µl | |
Σ | 10 µl |