Aug 29, 2024

Public workspaceSeizures – like behavior assay for D. melanogaster

DOI
dx.doi.org/10.17504/protocols.io.81wgbzqd1gpk/v1
  • 1VIB-KU Leuven Center for Brain & Disease Research, 3000 Leuven, Belgium.
Natalie Kaempf
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DOI: dx.doi.org/10.17504/protocols.io.81wgbzqd1gpk/v1
Protocol Citation: Natalie Kaempf, Patrik Verstreken 2024. Seizures – like behavior assay for D. melanogaster. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbzqd1gpk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 29, 2024
Last Modified: August 29, 2024
Protocol Integer ID: 104257
Keywords: ASAPCRN, seizure-like behavior
Funders Acknowledgement:
Aligning Science Across Parkinson’s
Grant ID: ASAP-000430
EMBO long-term postdoctoral fellowship
Grant ID: ALTF_299-2019
Research project, FWO Vlaanderen
Grant ID: G0A5219N
Research project, FWO Vlaanderen
Grant ID: G0B8119N
Methusalem project
Grant ID: METH/21/05 (3M210778)
Research project, KU Leuven Parkinson Fonds
Grant ID: EQZ-PARFON-O2010
Opening the Future grant, Leuvens Universiteitsfonds (LUF)
Grant ID: EQZ-OPTFUP-O2010
Research project, FWO Vlaanderen
Grant ID: G031324N
Abstract
This protocol allows to assess the susceptibility of flies to seizure-like behavior based on mechanically hyper-stimulating sensory inputs and assessing seizure-like activity.
Seizures – like behavior assay
Seizures – like behavior assay
30m
30m
prepare groups of 5 ± 1 flies and transfer them to transparent empty vials
let them rest for at least Duration00:30:00 after CO2 anesthesia

30m
mechanically stimulate the flies by vortexing the vial for 10 s at maximum intensity
move the vial immediatly on an even surface before a white background and start recording the behavior
Numbers of resistant flies and flies with seizure-like behavior are counted within 10 s post stimulation
Protocol references
Kuebler, D., and M. A. Tanouye. 2000. “Modifications of Seizure Susceptibility in Drosophila.” Journal of Neurophysiology 83 (2): 998–1009. https://doi.org/10.1152/jn.2000.83.2.998.