Dec 04, 2024

Public workspacescRNA-seq protocol

DOI
dx.doi.org/10.17504/protocols.io.q26g7my5kgwz/v1
  • 1Sloan Kettering Institute;
  • 2Johns Hopkins University
  • IGVF
Michael Beer
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DOI: dx.doi.org/10.17504/protocols.io.q26g7my5kgwz/v1
Protocol Citation: Renhe Luo, Michael Beer, Danwei Huangfu 2024. scRNA-seq protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7my5kgwz/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 04, 2024
Last Modified: December 04, 2024
Protocol Integer ID: 113707
Funders Acknowledgements:
NHGRI
Grant ID: U01 HG012051
Abstract
scRNA-seq protocol for ESC DE differentiation time course
scRNA-seq protocol
scRNA-seq protocol
Cells were harvested every 12h during DE differentiation for scRNA-seq experiments with targeted collection ranging from 3000 to 6000 cells.
Single-cell 3′ RNA-seq libraries were generated with 10x Genomics Chromium Single Cell 3′ Reagent Kit v.3 following the manufacturer’s guidelines using 10X chromium controller firmware v5.0.
The libraries were sequenced on NovaSeq 6000 platform following the manufacturer’s guidelines.