Place the cell plate into the IncuCyte live-cell analysis system and allow the plate to warm to 37°C for 30 minutes prior to scanning.
a. Objective: 4x, 10x (recommended), or 20x
b. Channel selection: Phase Contrast (+ Fluorescence if analyzing cells with fluorescent labels)
c. Scan type: Scratch Wound (Wide Mode optional for 10x, required for 20x)