Dec 15, 2022

Public workspaceScott's Tap Water Substitute

This protocol is a draft, published without a DOI.
  • 1UC Davis
joshdm m
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Protocol Citation: joshdm m 2022. Scott's Tap Water Substitute. protocols.io https://protocols.io/view/scott-39-s-tap-water-substitute-ckhrut56
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 14, 2022
Last Modified: December 15, 2022
Protocol Integer ID: 74001
Abstract
Used as a bluing agent in H&E staining
Protocol materials
ReagentMagnesium Sulfate HeptahydrateFisher ScientificCatalog #M63-500
Step 1
ReagentSodium BicarbonateMerck MilliporeSigma (Sigma-Aldrich)Catalog #s-5761
Step 2
Measure and mix salts
Measure and mix salts
weigh Amount20 g of ReagentMagnesium Sulfate HeptahydrateFisher ScientificCatalog #M63-500

weigh Amount2 g of ReagentSodium BicarbonateSigma AldrichCatalog #s-5761

Mix in ddWater until completely dissolved
store at room temperature
Ph8.3 to Ph8.7