Nov 19, 2024

Public workspaceSaturation Genome Editing

DOI
dx.doi.org/10.17504/protocols.io.rm7vzjyd4lx1/v1
  • 1University of Washington, Department of Genome Sciences, Brotman Baty Institute
  • Atlas of Variant Effects Alliance
Nahum Smith
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DOI: dx.doi.org/10.17504/protocols.io.rm7vzjyd4lx1/v1
Collection Citation: Nahum Smith, Silvia Casadei 2024. Saturation Genome Editing. protocols.io https://dx.doi.org/10.17504/protocols.io.rm7vzjyd4lx1/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: March 04, 2024
Last Modified: November 19, 2024
Collection Integer ID: 99550
Funders Acknowledgement:
NHGRI (IGVF)
Grant ID: HG011969
Abstract
This collection details the saturation genome editing workflow.
Guidelines
Data Analysis
  • Demultiplex samples from Illumina output directory using BCLConvert software.
Files
Protocol
Icon representing the file Design
Name
Design
Version 1
, University of Washington
Nahum SmithUniversity of Washington
Protocol
Icon representing the file Guide Cloning
Name
Guide Cloning
Version 1
, University of Washington
Nahum SmithUniversity of Washington
Protocol
Icon representing the file Library Cloning
Name
Library Cloning
Version 1
, University of Washington
Nahum SmithUniversity of Washington
Protocol
Icon representing the file Tissue culture
Name
Tissue culture
Version 1
, University of Washington
Nahum SmithUniversity of Washington
Protocol
Icon representing the file Pre-Sequencing
Name
Pre-Sequencing
Version 1
, University of Washington
Nahum SmithUniversity of Washington