Jan 22, 2024

Public workspaceSamuel Lab Media and Buffers

This collection is a draft, published without a DOI.
  • 1Baylor College of Medicine
Open access
Collection CitationAdrien Assie, Buck Samuel 2024. Samuel Lab Media and Buffers. protocols.io https://protocols.io/view/samuel-lab-media-and-buffers-zyxf7xn
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 10, 2019
Last Modified: January 22, 2024
Collection Integer ID: 22263
Disclaimer
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

Most of the protocols in this collection are derived from the wormbook protocol collection
Abstract
This is a collection of protocol for the preparation of standard media used in the Samuel Lab
Materials
1 M CaCl2 (Filter Sterilize)
5 mg/mL Cholesterol in 200 Proof Ethanol (Store at 4°C)
*500 mM EDTA, pH 8.0
5 M KOH
1 M MgSO­­4 (Autoclave)
5 M NaOH
*1 M Potassium Citrate Buffer, pH 6.0
*1 M Potassium Phosphate Buffer, pH 6.0
*Trace Metals Solution
DISCLAIMER – FOR INFORMATIONAL PURPOSES ONLY; USE AT YOUR OWN RISK

Most of the protocols in this collection are derived from the wormbook protocol collection
Files
Protocol
0.5M EDTA, pH 8.0
Name
0.5M EDTA, pH 8.0
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
1M Potassium Phosphate Buffer, pH 6.0
Name
1M Potassium Phosphate Buffer, pH 6.0
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
1M Potassium Citrate Buffer, pH 6.0
Name
1M Potassium Citrate Buffer, pH 6.0
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
M9 Medium
Name
M9 Medium
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
LB borth or agar
Name
LB borth or agar
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
NGM Agar
Name
NGM Agar
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
Mannitol Agar
Name
Mannitol Agar
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
Worm Freezing Buffer
Name
Worm Freezing Buffer
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
Trace Metals Solution
Name
Trace Metals Solution
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
S-Basal Medium
Name
S-Basal Medium
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine
Protocol
S-Complete Medium
Name
S-Complete Medium
Version 1
Adrien Assie
Adrien AssieBaylor College of Medicine