A | B | C | D | |
Tissue | Size§ | Conc. of Collagenase A¶ | Time of incubation† | |
Cornea | 4mm punch X 2 | 1.5 mg/ml | ~ 2 h | |
Limbus | 4mm punch X 2 | 2 mg/ml | ~ 2 h | |
Sclera | Bigger the better | 2.5 mg/ml | > 2 h | |
Ciliary body | 1/8 – 1/4 of all | 1 mg/ml | 30 min ~ 1 h | |
Iris | 1/4 - 1/2 of all | 1 mg/ml | < 30 min | |
TM | All | 1.5 mg/ml | ~1 h | |
Lens capsule | 1/2 - all | 1 mg/ml | < 30 min |
Tissue | Time of incubation | |
Cornea | ~ 1 h | |
Limbus | 30 min - 1 h | |
Ciliary body | ~ 30 min | |
Iris | ~ 30 min | |
Lens | < 30 min |