Feb 07, 2025

Public workspaceSample preparation for PIV in organ-on-a-chip device

DOI
dx.doi.org/10.17504/protocols.io.ewov1dme2vr2/v1
  • 1University of Latvia
Viesturs Sints
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DOI: dx.doi.org/10.17504/protocols.io.ewov1dme2vr2/v1
Protocol Citation: Viesturs Sints 2025. Sample preparation for PIV in organ-on-a-chip device. protocols.io https://dx.doi.org/10.17504/protocols.io.ewov1dme2vr2/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 07, 2025
Last Modified: February 07, 2025
Protocol Integer ID: 119793
Abstract
The protocol describes preparation of samples to be used in PIV measurements performed in OOC devices. Keep in mind that the substances and variables may change significantly depending on the optical system and OOC device used.
Prepare Amount1 mL Sampleflow substance

Take Amount50 µL of tracer particle solution (example: 1 μm Nile Red; must be chosen for the laser used in PIV and the optical system) and add to Sampleflow substance

Shake for Duration00:00:05 in a lab tube shaker

5s
Place for Duration00:30:00 in an ultrasonic bath

30m
The sample is now prepared for PIV measurements. It cannot be reliably expected to retain colloidal stability for more than Duration01:00:00