A | B | C | |
Item | Vendor | Catalog # | |
DMEM/F12 | Thermo Fisher | 11320082 | |
Knockout Serum Replacement | Thermo Fisher | 10828-028 | |
Newborn Calf Serum | Sigma | N4762 | |
L-Glutamine | Sigma | G8540 | |
Penicillin & Streptomycin (100X) | Thermo Fisher | 15140163 | |
MEM Non-Essential Amino Acids (100X) | Thermo Fisher | 11140050 | |
Collagenase type IV | Thermo Fisher | 17104019 | |
40 micron Cell Strainer | Corning | 352340 | |
DPBS w/o Calcium and magnesium (DPBS) | Corning | MT21031CV |
Collagenase type IV | 10 mg | |
KSR medium | 10 ml |
DMEM/F12 | 385 ml | |
Knockout Serum Replacement | 100 ml | |
L-Glutamine (200 mM) | 5 ml | |
Penicillin & Streptomycin (100X) | 5 ml | |
MEM Non-Essential Amino Acids (100X) | 5 ml |
DMEM/F12 | 470 ml | |
Newborn Calf Serum | 25 ml | |
Penicillin & Streptomycin (100X) | 5 ml |