A | B | |
Barcode Name | Sequence | |
SMRT-A_bc-none | /5Phos/CTG TCT CTT ATA CAC ATC TAT CTC TCT CTT TTC CTC CTC CTC CGT TGT TGT TGT TGA GAG AGA TAG ATG TGT ATA AGA GAC AG | |
SMRT-A_bc001 | /5Phos/CTG TCT CTT ATA CAC ATC TTT CTT CCG ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT CGG AAG AAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc003 | /5Phos/CTG TCT CTT ATA CAC ATC TTT CCA CAC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GTG TGG AAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc006 | /5Phos/CTG TCT CTT ATA CAC ATC TTT GTC GCA ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT TGC GAC AAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc010 | /5Phos/CTG TCT CTT ATA CAC ATC TTT AGC TGC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GCA GCT AAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc011 | /5Phos/CTG TCT CTT ATA CAC ATC TTC CTA AGG ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT CCT TAG GAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc012 | /5Phos/CTG TCT CTT ATA CAC ATC TTC CGT TGT ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT ACA ACG GAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc013 | /5Phos/CTG TCT CTT ATA CAC ATC TTC GAA TCG ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT CGA TTC GAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc014 | /5Phos/CTG TCT CTT ATA CAC ATC TTC ACT GTG ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT CAC AGT GAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc015 | /5Phos/CTG TCT CTT ATA CAC ATC TTG CAG GAT ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT ATC CTG CAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc016 | /5Phos/CTG TCT CTT ATA CAC ATC TTA TGG CGT ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT ACG CCA TAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc017 | /5Phos/CTG TCT CTT ATA CAC ATC TTA CCG ACT ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT AGT CGG TAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc018 | /5Phos/CTG TCT CTT ATA CAC ATC TTA CAA GCC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GGC TTG TAA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc019 | /5Phos/CTG TCT CTT ATA CAC ATC TCT GAC CAA ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT TTG GTC AGA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc020 | /5Phos/CTG TCT CTT ATA CAC ATC TCC TCT CTA ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT TAG AGA GGA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc021 | /5Phos/CTG TCT CTT ATA CAC ATC TCC TGT AAC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GTT ACA GGA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc022 | /5Phos/CTG TCT CTT ATA CAC ATC TCC GCA TAA ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT TTA TGC GGA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc023 | /5Phos/CTG TCT CTT ATA CAC ATC TCA AGT GGA ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT TCC ACT TGA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc024 | /5Phos/CTG TCT CTT ATA CAC ATC TGT GCA TTC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GAA TGC ACA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc025 | /5Phos/CTG TCT CTT ATA CAC ATC TGG CTT CAT ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT ATG AAG CCA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc026 | /5Phos/CTG TCT CTT ATA CAC ATC TGG AAC TAC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GTA GTT CCA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc027 | /5Phos/CTG TCT CTT ATA CAC ATC TGA CGT TAG ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT CTA ACG TCA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc028 | /5Phos/CTG TCT CTT ATA CAC ATC TGA GTG TCT ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT AGA CAC TCA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc029 | /5Phos/CTG TCT CTT ATA CAC ATC TGA AGA AGG ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT CCT TCT TCA GAT GTG TAT AAG AGA CAG | |
SMRT-A_bc030 | /5Phos/CTG TCT CTT ATA CAC ATC TAA CAC CTC ATC TCT CTC TTT TCC TCC TCC TCC GTT GTT GTT GTT GAG AGA GAT GAG GTG TTA GAT GTG TAT AAG AGA CAG |
A | B | C | D | |
Reagent (final conc.) | Stock conc. | Volume | Note | |
20 mM HEPES | 1M, pH 7.5 | 200µL | ||
10 mM KCl | 2M | 50µL | ||
1 mM MgCl2 | 1M | 10µL | ||
0.1% Triton X-100 | 10% | 100µL | ||
20% glycerol | 100% | 2mL | ||
1x Protease Inhibitor | 25x | 400µL | Add 25x Roche protease inhibitor immediately before use | |
dH2O | - | up to 10mL | ||
Total Volume | 10mL | |||
A | B | C | |
Reagent (final conc.) | Stock conc. | Volume | |
15mM Tris-HCl, pH 8.0 | 1M | 150µL | |
15mM NaCl | 5M | 30µL | |
60mM KCl | 2M | 300µL | |
0.5mM spermidine | 1M | 5µL | |
dH2O | - | up to 10mL | |
Total Volume | 10mL | ||
A | B | C | |
Reagent (final conc.) | Stock conc. | Volume | |
20mM HEPES pH 7.5 | 1M | 200µL | |
150mM NaCl | 5M | 300µL | |
0.25mM spermidine | 1M | 2.5µL | |
0.1X Protease Inhibitor | 25X | 40µL | |
dH2O | - | up to 10mL | |
Total Volume | 10mL | ||
A | B | C | D | |
Reagent (final conc.) | Stock conc. | Volume | Note | |
10mM Tris-HCl, pH 7.5 | 1M | 10µL | ||
5mM MgCl2 | 1M | 5µL | ||
10% DMF | 100% | - | Add directly into Omni-ATAC Reagent Mix at step 20 | |
dH2O | - | 185µL | ||
Total Volume | 200µL | |||
A | B | C | |
Reagent (final conc.) | Stock conc. | Volume (per sample) | |
1x TD premix | 5x | 5µL | |
0.33x PBS | 1x | 16.5µL | |
10% DMF | 100% | 5µL | |
0.01% digitonin | 1% | 0.5µL | |
0.1% Tween-20 | 10% | 0.5µL | |
dH2O | - | up to 50µL | |
Total Volume | 50µL | ||
A | B | C | |
Reagent (final conc.) | Stock conc. | Volume (per sample) | |
Proteinase K (6.66mg/mL) | 20mg/mL | 2.5µL | |
3.33% SDS | 10% | 2.5µL | |
167mM EDTA | 0.5M | 2.5µL | |
Total Volume | 7.5µL |
A | B | C | |
Reagent (final conc.) | Stock conc. | Volume (per sample) | |
tagmented sample (up to 160ng) | - | 12µL* | |
dNTP mix (0.8mM) | 8mM | 2µL | |
1x Taq DNA Ligase Rxn Buffer | 10x | 2µL | |
NEB Phusion (0.1U) | 2U/µL | 1µL | |
NEB Taq DNA Ligase (4U) | 40U/µL | 2µL | |
dH20 | - | 1µL | |
Total Volume | 20µL | ||
A | B | C | |
Reagent (final conc.) | Stock conc. | Volume (per sample) | |
gap-repaired sample | - | 12µL* | |
NEBuffer 2 (1x) | 10x | 1.5µL | |
Exonuclease III (100U) | 100U/µL | 1µL | |
dH20 | - | 0.5µL | |
Total Volume | 15µL | ||