License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: June 07, 2024
Last Modified: June 25, 2024
Protocol Integer ID: 102387
Keywords: ASAPCRN
Funders Acknowledgement:
ASAP
Grant ID: 000592
Abstract
This protocol details the clearance of αS PFF assay.
αS PFF Clearance Assay Protocol
αS PFF Clearance Assay Protocol
2h 1m
Prepare S PFF working solution as above (for 4 undetermined final concentration).
Treat for 02:00:00.
2h
Wash cells with PBS followed by trypsin (000.5%) for 00:01:00 to remove excess PFF bound on the extracellular surface.
1m
Incubate with fresh media in presence or absence of other drugs/inhibitor treatment for desired duration prior to lysate collection.