GoTaq® Enviro Wastewater SARS-CoV-2 System, N1 200 reactions Catalog Number: AM2110
2 × 100μl N1 & PMMoV Primer/Probe/IAC Mix, 20X
2 × 1,000μL GoTaq® Enviro Master Mix, 2X
1 × 100μL GoScript™ Enzyme Mix
2 × 1.25mL Nuclease-Free Water
1 × 100μL SARS-CoV-2 (N+E) RNA, 4 × 106 copies/μL
1 × 100μL PMMoV RNA, 4 × 106 copies/μL
Storage Conditions: Store all components of the GoTaq® Enviro Wastewater SARS-CoV-2 Systems at –30°C to –10°C. Limit freeze-thaws to five cycles or less. Store the 20X Primer/Probe/IPC mixes protected from light.
Materials to Be Supplied by User:
10% Bleach for cleaning workspaces
RNAseAway 7000TS1 (or similar)
Sterile aerosol-resistant barrier pipette tips
Pipettes dedicated to pre-amplification work
1.5mL microcentrifuge tubes to prepare the reaction mixes
0.5mL low-bind microcentrifuge tubes (e.g., Eppendorf Cat.# 022431005) to prepare the standard dilutions
qPCR plates (e.g. MicroAmp™ Optical 96-Well Reaction Plate Catalog Number: N8010560
Adhesive covers (e.g., MicroAmp™ Optical Adhesive Film 4311971) OR optical strip caps (e.g., MicroAmp™ Optical 8-Cap Strips 4323032)
ABI 7500 Fast qPCR thermal cycler (or similar)
Ice bucket & ice OR
Corning XT Ice-free Cooling Core 432014 & Corning™ CoolRack™ XT PCR Tube Module 432053 (or similar)
Centrifuge for microcentrifuge and reagent tubes
Centrifuge for 96-well plates