Primer name | Sequence (5′->3′) | |
RSVA-fragment 1-Fw | AAAAATGCGTACWACAAACTTGC | |
RSVA-fragment 1-Rev | GTTGGTCCTTGGTTTTGGAC | |
RSVA-fragment 2-Fw | CACAGTGACTGACAACAAAGGAG | |
RSVA-fragment 2-Rev | GCTCATGGCAACACATGC | |
RSVA-fragment 3-Fw | CGAGGTCATTGCTTGAATGG | |
RSVA-fragment 3-Rev | CACCACCACCAAATAACATGG | |
RSVA-fragment 4-Fw | AGGGTGGTGTCAAAAACTATGG | |
RSVA-fragment 4-Rev | ACGAGAAAAAAAGTGTCAAAAACT |
component | μl | |
primer F (10μM) | 0,5 | |
primer R (10μM) | 0,5 | |
SuperScript III RT/Platinum Taq Hi-Fidelity Enzyme Mix | 0,5 | |
2x reaction mix | 12,5 | |
template RNA | 5 | |
water | 6 |