Feb 28, 2024
- sdwalto1,
- Bryan Killinger2,
- Jeffrey Kordower1
- 1ASU NDRC;
- 2Rush University
Protocol Citation: sdwalto, Bryan Killinger, Jeffrey Kordower 2024. Rotarod. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgq3xmolk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 28, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95890
Keywords: ASAPCRN, rotarod, mice behavior
Funders Acknowledgement:
Jeffrey Kordower
Grant ID: NIH R21 NS109871
Abstract
Rotarod protocol optimized for mice. This test is made to assess motor coordination and balance. The results should show lower latency to fall numbers for mice with motor impairments.
Safety warnings
Depending on the rotarod apparatus, you need to be careful of mice tails being caught in-between the rotating rod and the separating wall panels.
Ethics statement
Animal maintenance and experiments were performed in accordance with the National Institutes of Health guidelines and approved by the Institutional Animal Care and Use Committee of the Rush University Medical Center (Chicago, IL).
Training: Each mouse was given a training session. Four 5 min trials, 5 mins apart.
Test Period (1 hour later): Each mouse was place inside the rotarod apparatus with increasing speed (4 rpm to 40 rpm in 300 secs). Latency to fall was recorded. (Digital rotarod recorded it automatically)
Each mouse received two consecutive trials. Mean latency to fall was used for analysis.