Apr 05, 2024
Version 2
Rotarod (40 RPM and 20 RPM with docking) V.2
- daniel.dautan daniel1,
- Per Svenningsson1
- 1Karolinska Institute Stockholm
Protocol Citation: daniel.dautan daniel, Per Svenningsson 2024. Rotarod (40 RPM and 20 RPM with docking) . protocols.io https://dx.doi.org/10.17504/protocols.io.3byl4qo5zvo5/v2Version created by Eileen Ruth Torres
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 22, 2024
Last Modified: May 31, 2024
Protocol Integer ID: 95631
Keywords: ASAPCRN
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: 020608
Abstract
This protocol describes motor function testing in mice using the rotarod by the Svenningsson lab.
Two options for the protocol are included: 1) 40 RPM constant speed after habituation and 2) 20 RPM with docking.
Materials
Mouse Rotarod (Ugo Basile 47650)
50% ethanol
Transfer the cage into the experimental room at least 1 hr before testing.
To avoid stress, always put the cage into a ventilated cage rack.
Using a paper tube, transfer each mouse of the same cage onto the rotarod (Ugo Basile, 47650). Always maintain order, mice one into line one, mice 2 into line 2 etc…
Wait 2-3 minutes for the mice to habituate to the apparatus.
Lift the collecting platform and set up the protocol: either max speed of constant 40 RPM or 20 RPM docking.
40 RPM4 steps
Following habituation, start the rotarod program to reach a speed of 40 RPM within the first 5s of the protocol.
The time to fall is defined as the moment the mice fall in the receptacle or the moment the mice turn around the rotor more than 5 consecutive times. The maximum trial length is 2 min.
Repeat the experiment 3 times with at least 6 hr between each trial. If an animal does not fall or turn more than 5 times within 120s of the trial, stop the trial and record “120” as the individual time.
Analyze for each mouse, the time to fall for each session, the average and and max time.