Aug 20, 2019
RNA Isolation from Plant Tissue Protocol 17: Invitrogen PureLink-Qiagen RNeasy Hybrid
Peer-reviewed method
- GigaScience Press
External link: https://doi.org/10.1093/gigascience/giz126
Protocol Citation: Eric Carpenter: RNA Isolation from Plant Tissue Protocol 17: Invitrogen PureLink-Qiagen RNeasy Hybrid. protocols.io https://dx.doi.org/10.17504/protocols.io.4uwgwxe
Manuscript citation:
Carpenter EJ, Matasci N, Ayyampalayam S, Wu S, Sun J, Yu J, Jimenez Vieira FR, Bowler C, Dorrell RG, Gitzendanner MA, Li L, Du W, K Ullrich K, Wickett NJ, Barkmann TJ, Barker MS, Leebens-Mack JH, Wong GK. Access to RNA-sequencing data from 1,173 plant species: The 1000 Plant transcriptomes initiative (1KP). Gigascience. 2019 Oct 1;8(10):giz126. doi: 10.1093/gigascience/giz126.
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
These protocols were used for RNA extraction from plant tissues in order to support the One Thousand Plants initiative's work to produce RNA-Seq transcriptomes from a diverse collection of plant samples.
Created: June 27, 2019
Last Modified: August 22, 2019
Protocol Integer ID: 25206
Keywords: RNA, RNA extraction, RNA isolation, plant tissue
Abstract
Implemented by: Patrick Edger and J. Chris Pires
For a small number of samples the Invitrogen PureLink™ RNA Mini Kit (Cat #12183-018A) was used to isolate total RNA while the Qiagen RNeasy MinElute Cleanup Kit (Cat #74204) was used to purify and concentrate total RNA. The methods followed the manufacturer’s instructions and thus they are not repeated here.
This protocol is part of a collection of eighteen protocols used to isolate total RNA from plant tissue. (RNA Isolation from Plant Tissue Collection: https://www.protocols.io/view/rna-isolation-from-plant-tissue-439gyr6)
Attachments
Materials
MATERIALS
RNeasy MinElute Cleanup KitQiagenCatalog #74204
PureLink™ RNA Mini KitThermo Fisher ScientificCatalog #12183020
Safety warnings
Please see SDS (Safety Data Sheet) for hazards and safety warnings.