Sep 13, 2024
RNA-interference in Paramecium bursaria
- 1University of Oxford
Collection Citation: Ben Jenkins 2024. RNA-interference in Paramecium bursaria. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv5jzm4l1b/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: October 11, 2022
Last Modified: September 13, 2024
Collection Integer ID: 71205
Keywords: Paramecium, bursaria, RNAi, gene, knock-down, reverse genetics, ciliate, algae, symbiosis
Abstract
RNA-interference based gene knock-down in Paramecium bursaria. This collection contains protocols for:
1) Paramecium cultures for RNAi - Filtering and preparing P. bursaria cultures for downstream RNAi
2) RNAi induction - Back-dilution, induction, and creating frozen E. coli feeding stocks for downstream RNAi
3) RNAi feeding - Daily resuspension and feeding of P. bursaria cultures
4) RNAi imaging - Imaging of P. bursaria cultures
Image Attribution
Ben Jenkins
