License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 04, 2022
Last Modified: March 04, 2022
Protocol Integer ID: 59053
Abstract
This protocol is used to extract RNA from mouse brain tissue and quantify with RT-qPCR.
Homogenize hemibrain in 3mL 1X PEPI buffer using a dounce homogenizer.