May 13, 2024

Public workspaceRNA extraction and PCR protocol

DOI
dx.doi.org/10.17504/protocols.io.81wgbz7r3gpk/v1
  • Enrico Bagnoli1,2,
  • Miratul Muqit1,2
  • 1Aligning Science Across Parkinson’s;
  • 2Medical Research Council Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences, University of Dundee, Dow Street, Dundee DD1 5EH, UK
Francesca Tonelli
Open access
DOI: dx.doi.org/10.17504/protocols.io.81wgbz7r3gpk/v1
Collection Citation: Enrico Bagnoli, Miratul Muqit 2024. RNA extraction and PCR protocol. protocols.io https://dx.doi.org/10.17504/protocols.io.81wgbz7r3gpk/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 23, 2024
Last Modified: May 13, 2024
Collection Integer ID: 98709
Keywords: ASAPCRN
Funders Acknowledgement:
ASAP
Grant ID: ASAP-000463
Abstract
This protocol details the extraction of RNA and PCR analysis.
Files
Protocol
RNA extraction using the PureLink® RNA Mini Kit
Name
RNA extraction using the PureLink® RNA Mini Kit
Version 1
Francesca Tonelli
Francesca TonelliMRC-PPU at The University of Dundee
Protocol
cDNA synthesis using the QuantiTect Reverse transcription kit
Name
cDNA synthesis using the QuantiTect Reverse transcription kit
Version 1
Francesca Tonelli
Francesca TonelliMRC-PPU at The University of Dundee
Protocol
PCR and analysis
Name
PCR and analysis
Version 1
Francesca Tonelli
Francesca TonelliMRC-PPU at The University of Dundee