Jul 13, 2023
Ringer's Solution
- Mark H. Ellisman1,
- Mason Mackey1,
- Eric Bushong1,
- Marianne Martone1,
- Thomas Deerinck1
- 1National Center for Microscopy and Imaging Research, University of California, San Diego, La Jolla CA
Protocol Citation: Mark H. Ellisman, Mason Mackey, Eric Bushong, Marianne Martone, Thomas Deerinck 2023. Ringer's Solution. protocols.io https://dx.doi.org/10.17504/protocols.io.8epv59e16g1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 20, 2022
Last Modified: July 13, 2023
Protocol Integer ID: 61105
Keywords: ringers, perfusion, mice, Lidocaine, Heparin, salts, solution
Abstract
Standard protocol for preparing 100mL of Ringer's Solution for mice and rat perfusions.
Guidelines
Make sure you bubble for at least 5 minutes before adding calcium chloride to Ringer's solution to prevent precipitation. If precipitation occurs, discard and remake entire solution.
Materials
- 100mL Erlenmeyer flask
- 1000μL Pipette
- Serological Pipettes
Safety warnings
Always wear appropriate PPE.
1.0 mL (Na2HPO4, 18 g/L, at room temperature)
9.9 mL (NaCl, 79.8 g/L, 4 ºC fridge)
1.0 mL (KCl, 37.5 g/L, 4 ºC fridge)
1.0 mL (MgCl2• 6H2O, 20 g/L, 4 ºC fridge)
2.5 mL (NaHCO3, 50 g/L, 4 ºC fridge)
0.200g of Dextrose
Bring solution up to 97ml with double distilled water (ddH2O).
1.0 ml Lidocaine hydrochloride
1.0 ml heparin (prevents blood clotting)
—bubble 95% air: 5% CO2 at 35 ºC
1.0 mL (CaCl2• 2H2O, 30 g/L, 4 ºC fridge)