Jul 23, 2020
Retro-orbital Bleeding for Rats
This protocol is a draft, published without a DOI.
- 1University of California, San Diego
- George Lab @ UCSDTech. support email: olgeorge@ucsd.edu
Protocol Citation: Sharona Sedighim, Olivier George 2020. Retro-orbital Bleeding for Rats. protocols.io https://protocols.io/view/retro-orbital-bleeding-for-rats-bie4kbgw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: July 09, 2020
Last Modified: July 23, 2020
Protocol Integer ID: 39100
Eye Bleeds Procedure
Eye Bleeds Procedure
Take a microcapillary tube (broken in half) and place it behind the eye.
Twist it until you hear/feel a little pop, and blood should start coming out.
Let blood drip into the purple collection tube (try to fill it to the top line).
Once you complete the collection, remove the microcapillary tube and clean the eye with a cotton tip applicator and/or gauze.
Put the cap on the tube, and slowly flip tube up and down so the blood goes on all sides of the tube (prevents it from clotting).