Oct 18, 2023
Respiratory rate (microCT)
- 1Vall d'Hebron Research Institute
Protocol Citation: Miquel Vila 2023. Respiratory rate (microCT). protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr3mm2vmk/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: October 18, 2023
Last Modified: November 20, 2023
Protocol Integer ID: 89558
Abstract
Respiratory rate (microCT) measurement in mice
For the scanning procedure, anestethize the animals with isoflurane (5% induction phase, 1% maintenance). Set the air flow to 0.8l/min.
Define parameters for image acquisition (field of view: 73 mm, acquisition time: 2 min, current voltage:70 mV, and amperage:200 uA).
Determine respiration frequency using microCT measurements acquired with Quantum FX imaging system (Perkin Elmer. 940 Winter St. Waltham, Massachusetts. EEUU).