In this protocol, total lipids from miroalgae is extracted with a mixture of water and Folch solvent (2:1 chloroform-methanol v/v). Filter and cell debris is commonly removed by filtration, which is laborious and time consuming. It is also the main reason to either cause sample loss and therefore underestimation; or contamination from filtration system and therefore overestimation. We now use centrifugation to remove filter debris, which stays in between the organic phase and water phase. The extracted lipids is dried under N2 gas flow, stored under -80 ºC for further measurement.