Check desired settings in the PACEtomo.py and PACEtomo_selectTargets.py scripts.
No need to change most of the things. Check to make sure:
zeroExpTime is set to 0, unless hybrid scheme is desired.
startTilt and max tilt can be changed later.
minDefocus, maxDefocus can be -3 to -5 but can also modify later
delayIS and delayTilt should be 0.5 or even 1
taOffsetPos is something like 0.8, based on Josh’s benchmarking on our Titan1 to deal with systematic defocus increase at high tilt
In PACEtomo_selectTargets.py:
Guidance is true for guided anchormap routine
beamDiameter should be something like 1.8 for 42x and suggested dose
maxTilt is ~60° for a 37-tilt tomogram