Mar 01, 2022
Quick Decapitation for Paraffin Embedding
- 1University of Ottawa
Protocol Citation: Haley Geertsma 2022. Quick Decapitation for Paraffin Embedding. protocols.io https://dx.doi.org/10.17504/protocols.io.b5szq6f6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: March 01, 2022
Last Modified: March 01, 2022
Protocol Integer ID: 58937
Abstract
This protocol is used to quickly decapitate mice and isolate their brain for paraffin embedding and microtome sectioning.
Sedate mice with isoflurane then quickly decapitate.
5m
Extract the brain and incubate in 4% paraformaldehyde or 10% formalin for 72 hours
3d
Incubate brain in 70% ethanol and submit for paraffin embedding. Once embedded, section brains at 5μm.
1h