Quick and dirty microbial genome extraction protocol using Edwards buffer.
I've been using this method for in-lab Archaeal evolution study for the last four years or so and it's been working fantastically well, especially for short read sequencing.
Certain microbes (such as Deinococcus) can be difficult to process properly using just this protocol - standard caveats and fixes tend to improve the output, such as adding initial lysozyme incubation step in the very beginning, sometimes combined with a freeze-thaw cycle for especially difficult samples (I'll update and upload a separate version of this protocol for difficult microbes in the future).
This particular protocol is aimed at getting as much intact, long-read capable DNA out from a microbe as cheaply as possible, as shown in the included gel picture.
Ideation and initial testing performed with Sebastian S. Cocioba at Binomica Labs for ONT Deinococcus radiophilus genome sequencing project, using RAD004 rapid sequencing kit.