A | B | |
Tris-HCl pH 7.5 | 50 mM | |
Triton X-100 | 1%(v/v) | |
EGTA | 1 mM | |
Na3VO4** | 1 mM | |
NaF | 50 mM | |
β-glycerophosphate | 10 mM | |
sodium pyrophosphate | 5 mM | |
sucrose | 0.27 M | |
cOmpleteTM, EDTA-free Protease Inhibitor Cocktail (Roche, 11836170001)** | ||
Microcystin-LR (Enzo Life Sciences, ALX-350-012)** |
A | B | |
Tris-HCl, pH6.8 | 250mM | |
SDS | 8% (w/v) | |
glycerol | 40% (v/v) | |
bromophenol blue | 0.02% (w/v) |
A | B | C | |
For NuPAGETM Bis-Tris gels | NuPAGE MOPS SDS running buffer (ThermoFisherScientific, Cat#NP000102) | ||
For self-cast Bis-Tris gels | MOPS | 50 mM | |
Tris | 50 mM | ||
SDS | 0.1% (w/v) | ||
EDTA | 1 mM |
A | B | |
Tris-HCl | 48 mM | |
glycine | 39 mM | |
freshly supplemented with 20% Methanol (v/v) |
A | B | |
Tris–HCl, pH 7.5 | 50 mM | |
NaCl | 150 mM | |
Tween 20 | 0.1% (v/v) |
A | B | C | D | E | |
Antibody Target | Company | Cat. number | Host species | Dilution | |
pS72 Rab7A | Abcam Inc. | MJF-38, Clone 1 | Rabbit | 1 ug/ml | |
Rab7A (Total) | Sigma | R8779 | Mouse | 1 ug/ml | |
pT202/Y204 ERK1/2 | Cell Signalling Technology | #9101 | Rabbit | 1 ug/ml | |
Phospho-(Ser) PKC Substrate Antibody | Cell Signalling Technology | #2261 | Rabbit | 1 ug/ml | |
LRRK1 (total) (C-terminus) | MRC-PPU Reagents and Services, University of Dundee | S405C | Sheep | 1 ug/ml | |
GAPDH | Antibodies Inc./NeuroMab | sc-32233 | Mouse | 1:5,000 | |
alpha-tubulin | Cell Signaling Technology | 3873 | Mouse | 1:5,000 |
A | B | C | D | |
Secondary Antibodies | Company | Cat. number | Notes | |
goat anti-mouse IRDye 680LT | LI-COR | 926-68020 | ||
goat anti-mouse IRDye 800CW | LI-COR | 926-32210 | ||
goat anti-rabbit IRDye 800CW | LI-COR | 926-32211 | ||
donkey anti-mouse IRDye 680LT | LI-COR | 926-68022 | ||
donkey anti-mouse IRDye 800CW | LI-COR | 926-32212 | ||
donkey anti-rabbit IRDye 800CW | LI-COR | 926-32213 | ||
donkey anti-goat IRDye 800CW | LI-COR | 926-32214 | Reacts with Sheep primary Abs |