Jun 05, 2023

Public workspaceQuantification of TH immunoreactivity

DOI
dx.doi.org/10.17504/protocols.io.n2bvj85qngk5/v1
  • 1Van Andel Institute;
  • 2ASAP
Johnson Agniswamy
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DOI: dx.doi.org/10.17504/protocols.io.n2bvj85qngk5/v1
Protocol Citation: Hong-Yuan Chu, Hong-Yuan Chu 2023. Quantification of TH immunoreactivity. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj85qngk5/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 31, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 82687
Keywords: Quantification of TH immunoreactivity, ASAPCRN
Funders Acknowledgement:
ASAP
Grant ID: ASAP-020572
Abstract
This protocol describes quantification of TH immunoreactivity.
Attachments
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731-1842.docx
13KB
Materials
Apparatus

  1. Confocal microscope
  2. Image J software
Procedure
Procedure
TH signals from bilateral striatum and cortex are imaged under a 20x objective lens using identical imaging settings.
Open the confocal files in Image J software.
Using the “Rectangle” function to select an area (width x high = 150 x 150) in striatum sections from both hemispheres for TH quantification.
Using the “Measure” function to measure the gray value of the selected area and write down the mean value of striatum slice, Mstriatum-LHS and Mstriatum-RHS.
Using the “Rectangle” function to select an area (width x high = 150 x 150) in cortical sections from both hemispheres for TH quantification.
Using the “Measure” function to measure the gray value of the selected area and write down the mean value of cortical slice, Mcortex-LHS and Mcortex-RHS.
TH quantification = .