After you have washed the Source 15S column with distilled water, you also need to wash the column with 100%
S-300 Buffer (high salt) to get rid of any remaining junk on the column. To this as follows:
1. Insert buffer lines A and B into your container of S-300 Buffer.
2. In the FPLC program, go to the "Manual" dropdown menu and select "1. Pump".
3. Flow --> Change to 1.00 mL / min
5. Change to "Gradient" --> Target: 100% B; Length: 0.10 min
7. Manual --> Pump --> Pump Wash
8. Turn Pump A on and Pump B on
10. Manual --> Other --> End Timer
11. Set timeout to 12.0 minutes
13. Sanity Check: Flow: 1.00; Gradient: 100, 0.10; Pump Wash on, on; End_Timer Acc time, 12:0