Apr 01, 2022
Purification of lysosomes using dextran-conjugated SPIONs
- William Hancock-Cerutti1,2,3,4,5,
- Arun Kumar Tharkeshwar1,
- Shawn Ferguson1,5,
- Pietro De Camilli1,2,3,5
- 1Departments of Neuroscience and of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
- 2Howard Hughes Medical Institute;
- 3Program in Cellular Neuroscience, Neurodegeneration and Repair, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
- 4Interdisciplinary Neuroscience Program and MD-PhD Program, Yale University School of Medicine, New Haven, Connecticut 06510, USA;
- 5Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD, 20815
Protocol Citation: William Hancock-Cerutti, Arun Kumar Tharkeshwar, Shawn Ferguson, Pietro De Camilli 2022. Purification of lysosomes using dextran-conjugated SPIONs. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l61dr1vqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: April 01, 2022
Last Modified: May 31, 2024
Protocol Integer ID: 60196
Keywords: ASAPCRN
Abstract
This method describes the usage of dextran-conjugated superparamagnetic iron nanoparticles (SPIONs) to purify a lysosome fraction from cultured cells.
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