A | B | |
Tris-HCl, pH 7.4 | 50 mM | |
NaCl | 300 mM | |
MgCl2 | 2 mM | |
Glycerol | 5% | |
Imidazole | 10 mM | |
β-mercaptoethanol | 2 mM |
A | B | |
Tris-HCl, pH 7.4 | 50 mM | |
NaCl | 300 mM | |
Imidazole | 10 mM | |
β-mercaptoethanol | 2 mM |
A | B | |
Tris-HCl, pH 7.4 | 25 mM | |
NaCl | 150 mM | |
DTT | 1 mM |
A | B | |
Tris-HCl, pH 7.4 | 50 mM | |
NaCl | 300 mM | |
MgCl2 | 2 mM | |
Glycerol | 5% | |
Imidazole | 10 mM | |
β-mercaptoethanol | 2 mM |
A | B | |
Tris-HCl, pH 7.4 | 50 mM | |
NaCl | 300 mM | |
Imidazole | 10 mM | |
β-mercaptoethanol | 2 mM |
A | B | |
Tris-HCl, pH 7.4 | 25 mM | |
NaCl | 150 mM | |
DTT | 1 mM |