Ultrapure DNase/RNase free dH2O (Thermofisher – Cat# 10-977-015)
RNA cleanup and concentrator (Zymo Research – Cat# R1015) or RNeasy MinElute Cleanup Kit (Qiagen – Cat# 74204)
QIAamp Viral RNA Mini Kit (Qiagen– Cat# 52904)
RNase-Free DNase Set (Qiagen– Cat# 79254)
100% Ethanol (we use VWR Cat#89125-170, but others will work)
iScript Reverse Transcription Supermix (BioRad - Cat #1708841)
dNTPs (we use Thermofisher – Cat#FERR0181, but others should work) – make a working stock of 25 mM
Taq DNA polymerase with standard Taq buffer (New England Biolabs – Cat#M0273)
Primers that bind on either side of the insertion/deletion/mutation