Encephalitozoon intestinalis (ATCC: 50506)
Vero cells (ATCC CCL-81)
DMEM:HG (Dulbecco's Modified Eagle Medium, high glucose)(Gibco 11995065)
FBS (Fetal Bovine Serum) (VWR 89510-188)
Non Essential Amino Acids (Gibco 11140050)
L-Glutamine, pH 6 to 8 (Gibco 25030081)
T-75 flask (CELLTREAT - 229341)
Cell scraper, 30 mm (CELLTREAT - 229315)
Percoll, pH range 8.5-9.5 (Cytiva 17-0891-02)
Sterile PBS, without calcium, without magnesium: 137.9 mM Sodium Chloride, 1.47 mM Potassium Phosphate Monobasic, 2.67 mM Potassium Chloride, 8.09 mM Sodium Phosphate Dibasic, pH 7.4±0.2
Ultracentrifuge tube (Thermo Scientific Cat #06752: 12 mL PET)
20% (w/v) SDS solution
Swinging bucket ultracentrifuge rotor (TH-641, Thermo Scientific Cat # 54295)
15 mL centrifuge tube (VWR 89039-664)
50 mL centrifuge tube (VWR 89039-656)
3 mL BD Luer-Lok Syringe sterile (BD 309657)
27G x 1/2 (0.4mm x 13mm) BD PrecisionGlide Needle (BD 305109)
BIOFlex HC Swinging-Bucket Rotor (Thermo Scientific Cat # 75003000)
Sorvall X4 Centrifuge (Thermo Scientific Cat # 75016052)
Fisherbrand Cover Glasses: circles (Cat#: 12-541-013)
Fisherbrand Selectfrost Microscope Slides (Cat#: 12-550-003)
Humidity chamber (Falcon 150mm x 15mm Not TC-treated Bacteriological Petri Dish (cat#:351058) lined with a damp paper towel)
Life Technologies prolong Diamond Antifade 1 (Cat#: P36965)
E. intestinalis 16S rRNA-specific FISH probe conjugated to Quasar 570 (LGC Biosearch Technologies). Oligonucleotide mix: tagtcccagagaatagcatc, tgtcaaaaacactcctagcc, taggcctacaacttacttgg, actcacttctcataaccgcc, gactcctttacactcgaaaa, caacactatatagtcccgtc, cccacatactcaagatttct, atccatttcaaacggccatg, cccaaagagcactgtcaaag, gggacccattcaaaacaact
Hybridization buffer (FISH):