;Rapid light curve in Actinic light 2 for TOMI-2
;version November 11, 2020
;high-resolution CCD - TOMI-2
;estimated time: app. 372 s
;for successful measurement check Light curve settings manual
include default.inc ;Includes standard options, do not remove it !
include light.inc ;Includes standard options, do not remove it !
include FW.inc ;Includes standard options, do not remove it !
;These are the light intensities used for the Rapid light curve
;_____________________________________________________________________________________________________________
;There is a pre-illumination phase with only far red light, followed by determination of Fo and Fm
<0s>=>add2(Preillumination)
;-------------------------------------------------------------------------------------------------------------
;*** Fo Measurement ******************************************************************************************
;-------------------------------------------------------------------------------------------------------------
start + <0,F0measure..F0duration>=>mfmsub
start + <0s>=>checkPoint,"startFo"
start + =>checkPoint,"endFo"
;-------------------------------------------------------------------------------------------------------------
;*** Saturating Pulse & Fm Measurement ***********************************************************************
;-------------------------------------------------------------------------------------------------------------
Fmstart = start + F0duration+mfmsub_length
=>SatPulse(PulseDuration)
Fmstart+<200ms, 200ms + mfmsub_length .. PulseDuration-mfmsub_length>=>mfmsub
=>checkPoint,"timeVisual"
;*** Actinic light Exposure *******************************
;Protocol contains 5 minutes of illumination with low light in order to keep photosynthesis active while samples are brogth to target temperatures by the thermocycler
ALstart1 = Fmstart + PulseDuration + Pause
ALstart2 = ALstart1 + ALduration + Pause
ALstart3 = ALstart2 + ALduration + Pause
ALstart4 = ALstart3 + ALduration + Pause
ALstart5 = ALstart4 + ALduration + Pause
ALstart6 = ALstart5 + ALduration + Pause
ALstart7 = ALstart6 + ALduration + Pause
ALstart8 = ALstart7 + ALduration + Pause
ALstart9 = ALstart8 + ALduration + Pause
ALstart10 = ALstart9 + ALduration + Pause
;this is where the Light Curve starts
ALstart11 = ALstart10 + ALduration + Pause
ALstart12 = ALstart11 + ALduration + Pause
ALstart13 = ALstart12 + ALduration + Pause
ALstart14 = ALstart13 + ALduration + Pause
ALstart15 = ALstart14 + ALduration + Pause
ALstart16 = ALstart15 + ALduration + Pause
ALstart17 = ALstart16 + ALduration + Pause
ALstart18 = ALstart17 + ALduration + Pause
ALstart19 = ALstart18 + ALduration + Pause
ALstart20 = ALstart19 + ALduration + Pause
ALstart21 = ALstart20 + ALduration + Pause
ALstart22 = ALstart21 + ALduration + Pause
ALstart23 = ALstart22 + ALduration + Pause
ALstart24 = ALstart23 + ALduration + Pause
ALstart25 = ALstart24 + ALduration + Pause
ALstart => act2(ALduration)
;******* Kautsky Effect Measurement **********************************
;******************* Ft'& Fm' definition **********************************
PulseLStart = ALduration - PulseDuration - mfmsub_length
FtL = ALstart + PulseLStart - PulseDuration
PulseL = ALstart + PulseLStart
PulseL - TS=> SI_Act2(I25)
PulseL=>SatPulse(PulseDuration)
PulseL#<200ms, 200ms + mfmsub_length .. PulseDuration-mfmsub_length>=>mfmsub
Last = ALstart25+ALduration+2*mfmsub_length
Last + <0s, 1s .. 5s>=>mfmsub
;--------------------------------------------------------------
=>checkPoint,"startFm_Lss1"
=>checkPoint,"endFm_Lss1"
=>checkPoint,"startFm_Lss2"
=>checkPoint,"endFm_Lss2"
=>checkPoint,"startFm_Lss3"
=>checkPoint,"endFm_Lss3"
=>checkPoint,"startFm_Lss4"
=>checkPoint,"endFm_Lss4"
=>checkPoint,"startFm_Lss5"
=>checkPoint,"endFm_Lss5"
=>checkPoint,"startFm_Lss6"
=>checkPoint,"endFm_Lss6"
=>checkPoint,"startFm_Lss7"
=>checkPoint,"endFm_Lss7"
=>checkPoint,"startFm_Lss8"
=>checkPoint,"endFm_Lss8"
=>checkPoint,"startFm_Lss9"
=>checkPoint,"endFm_Lss9"
=>checkPoint,"startFm_Lss10"
=>checkPoint,"endFm_Lss10"
=>checkPoint,"startFm_Lss11"
=>checkPoint,"endFm_Lss11"
=>checkPoint,"startFm_Lss12"
=>checkPoint,"endFm_Lss12"
=>checkPoint,"startFm_Lss13"
=>checkPoint,"endFm_Lss13"
=>checkPoint,"startFm_Lss14"
=>checkPoint,"endFm_Lss14"
=>checkPoint,"startFm_Lss15"
=>checkPoint,"endFm_Lss15"
=>checkPoint,"startFm_Lss16"
=>checkPoint,"endFm_Lss16"
=>checkPoint,"startFm_Lss17"
=>checkPoint,"endFm_Lss17"
=>checkPoint,"startFm_Lss18"
=>checkPoint,"endFm_Lss18"
=>checkPoint,"startFm_Lss19"
=>checkPoint,"endFm_Lss19"
=>checkPoint,"startFm_Lss20"
=>checkPoint,"endFm_Lss20"
=>checkPoint,"startFm_Lss21"
=>checkPoint,"endFm_Lss21"
=>checkPoint,"startFm_Lss22"
=>checkPoint,"endFm_Lss22"
=>checkPoint,"startFm_Lss23"
=>checkPoint,"endFm_Lss23"
=>checkPoint,"startFm_Lss24"
=>checkPoint,"endFm_Lss24"
=>checkPoint,"startFm_Lss25"
=>checkPoint,"endFm_Lss25"
;******************* Ft' definition **********************************
=>checkPoint,"startFt_Lss1"
=>checkPoint,"endFt_Lss1"
=>checkPoint,"startFt_Lss2"
=>checkPoint,"endFt_Lss2"
=>checkPoint,"startFt_Lss3"
=>checkPoint,"endFt_Lss3"
=>checkPoint,"startFt_Lss4"
=>checkPoint,"endFt_Lss4"
=>checkPoint,"startFt_Lss5"
=>checkPoint,"endFt_Lss5"
=>checkPoint,"startFt_Lss6"
=>checkPoint,"endFt_Lss6"
=>checkPoint,"startFt_Lss7"
=>checkPoint,"endFt_Lss7"
=>checkPoint,"startFt_Lss8"
=>checkPoint,"endFt_Lss8"
=>checkPoint,"startFt_Lss9"
=>checkPoint,"endFt_Lss9"
=>checkPoint,"startFt_Lss10"
=>checkPoint,"endFt_Lss10"
=>checkPoint,"startFt_Lss11"
=>checkPoint,"endFt_Lss11"
=>checkPoint,"startFt_Lss12"
=>checkPoint,"endFt_Lss12"
=>checkPoint,"startFt_Lss13"
=>checkPoint,"endFt_Lss13"
=>checkPoint,"startFt_Lss14"
=>checkPoint,"endFt_Lss14"
=>checkPoint,"startFt_Lss15"
=>checkPoint,"endFt_Lss15"
=>checkPoint,"startFt_Lss16"
=>checkPoint,"endFt_Lss16"
=>checkPoint,"startFt_Lss17"
=>checkPoint,"endFt_Lss17"
=>checkPoint,"startFt_Lss18"
=>checkPoint,"endFt_Lss18"
=>checkPoint,"startFt_Lss19"
=>checkPoint,"endFt_Lss19"
=>checkPoint,"startFt_Lss20"
=>checkPoint,"endFt_Lss20"
=>checkPoint,"startFt_Lss21"
=>checkPoint,"endFt_Lss21"
=>checkPoint,"startFt_Lss22"
=>checkPoint,"endFt_Lss22"
=>checkPoint,"startFt_Lss23"
=>checkPoint,"endFt_Lss23"
=>checkPoint,"startFt_Lss24"
=>checkPoint,"endFt_Lss24"
=>checkPoint,"startFt_Lss25"
=>checkPoint,"endFt_Lss25"
;END ******************************************************************