Feb 07, 2024

Public workspaceProtocols from Kilfeather, Khoo et al., 2024 V.2

  • 1University of Oxford
Open access
Collection CitationPeter Kilfeather, Maria Claudia Caiazza 2024. Protocols from Kilfeather, Khoo et al., 2024. protocols.io https://dx.doi.org/10.17504/protocols.io.36wgqj75kvk5/v2Version created by Peter Kilfeather
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 07, 2024
Last Modified: February 07, 2024
Collection Integer ID: 94822
Funders Acknowledgement:
Aligning Science Across Parkinson's
Grant ID: ASAP-020370
Parkinson's UK
Grant ID: J-1403
Science, Technology and Innovation Commission of Shenzhen Municipality
Grant ID: JCYJ20180507183628543
Abstract
This protocol collection includes immunohisto/chemical staining methods, DAT-TRAP methods and the protocol for the Fura-2 assay in Kilfeather, Khoo et al., 2024.
Files
Protocol
Immunocytochemistry for CASR in iPSc-derived dopaminergic neurons
Name
Immunocytochemistry for CASR in iPSc-derived dopaminergic neurons
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Fura-2 imaging of ionomycin response, with and without R568; a CaSR positive modulator
Name
Fura-2 imaging of ionomycin response, with and without R568; a CaSR positive modulator
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Immunohistochemistry
Name
Immunohistochemistry
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
DAT-TRAP Protocol
Name
DAT-TRAP Protocol
Version 2
Peter Kilfeather
Peter Kilfeather
Protocol
Stereo-seq cell type abundance analysis
Name
Stereo-seq cell type abundance analysis
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Differential gene expression analysis
Name
Differential gene expression analysis
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
GWAS prioritization analysis
Name
GWAS prioritization analysis
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Spatially variable gene detection
Name
Spatially variable gene detection
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Processing of raw Stereo-seq data, quality control and cell type identification
Name
Processing of raw Stereo-seq data, quality control and cell type identification
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Specificity index (pSI) calculation
Name
Specificity index (pSI) calculation
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Differential transcript usage analysis
Name
Differential transcript usage analysis
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Gene set enrichment analysis
Name
Gene set enrichment analysis
Version 1
Peter Kilfeather
Peter Kilfeather
Protocol
Long-read sequencing and data processing
Name
Long-read sequencing and data processing
Version 1
Peter Kilfeather
Peter Kilfeather