This is a collection of protocols from the manuscript: Ranoa, D. R. E.; Sharma, P.; Schane, C.P.; Lewis, A.N.; Valdez, E.; Marada, V. V. V. R.; Hager, M.; Montgomery, W.; Wolf, S. E.; Schreiber, K.; Schreiber, H.; Bailey, K.; Fan, T. M.; Hergenrother, P. J.; Roy, E. J.; Kranz, D. M. A single CAR-T cell treatment controls disseminated ovarian cancer in a syngeneic mouse model. Submitted to Journal for ImmunoTherapy of Cancer 2023; doi:10.1136/jitc-2022-006509.