May 24, 2023

Public workspaceProtocols from 237 CAR-T studies in a mouse ovarian tumor model (reported in Ranoa et al JITC 2023)

  • 1University of Illinois at Urbana-Champaign
Icon indicating open access to content
QR code linking to this content
Collection CitationDiana Rose E Ranoa, Preeti Sharma, Claire P. Schane, Amber N Lewis, Edward Valdez, Edward J. Roy, David M. Kranz 2023. Protocols from 237 CAR-T studies in a mouse ovarian tumor model (reported in Ranoa et al JITC 2023). protocols.io https://dx.doi.org/10.17504/protocols.io.6qpvr4jwpgmk/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: May 08, 2023
Last Modified: May 24, 2023
Collection Integer ID: 81610
Funders Acknowledgement:
David M Kranz
Grant ID: NIH CA238628
Abstract
This is a collection of protocols from the manuscript: Ranoa, D. R. E.; Sharma, P.; Schane, C.P.; Lewis, A.N.; Valdez, E.; Marada, V. V. V. R.; Hager, M.; Montgomery, W.; Wolf, S. E.; Schreiber, K.; Schreiber, H.; Bailey, K.; Fan, T. M.; Hergenrother, P. J.; Roy, E. J.; Kranz, D. M. A single CAR-T cell treatment controls disseminated ovarian cancer in a syngeneic mouse model. Submitted to Journal for ImmunoTherapy of Cancer 2023; doi:10.1136/jitc-2022-006509.
Files
Protocol
Icon representing the file  Isolation, activation, and retroviral transduction of primary T cells from murine splenocytes
Name
Isolation, activation, and retroviral transduction of primary T cells from murine splenocytes
Version 1
, University of Illinois at Urbana-Champaign
Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign
Protocol
Icon representing the file Protocols for processing of fresh murine tissues for flow cytometry
Name
Protocols for processing of fresh murine tissues for flow cytometry
Version 1
, University of Illinois at Urbana-Champaign
Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign
Protocol
Icon representing the file Immunohistochemistry of tissue sections from formalin-fixed paraffin embedded (FFPE) samples
Name
Immunohistochemistry of tissue sections from formalin-fixed paraffin embedded (FFPE) samples
Version 1
, University of Illinois at Urbana-Champaign
Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign
Protocol
Icon representing the file SRB assay for measuring target cell killing
Name
SRB assay for measuring target cell killing
Version 1
, University of Illinois at Urbana-Champaign
Diana Rose E RanoaUniversity of Illinois at Urbana-Champaign