Feb 09, 2023

Public workspaceProtocols for the assembly and annotation of snake genomes V.2

  • Boyang Liu1,
  • Liangyu Cui1,
  • Zhangwen Deng2,
  • Yue Ma1,
  • Diancheng Yang3,4,
  • Yanan Gong3,4,
  • Yanchun Xu1,
  • Shuhui Yang1,
  • Song Huang3,4
  • 1College of Wildlife and Protected Area, Northeast Forestry University, Harbin 150040, China;
  • 2Guangxi Forest Inventory and Planning Institute, Nanning 530011, China;
  • 3Anhui Province Key Laboratory of the Conservation and Exploitation of Biological Resource, College of Life Sciences, Anhui Normal University, Wuhu 241000, China;
  • 4Huangshan Noah Biodiversity Institute, Huangshan 245000, China
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Collection CitationBoyang Liu, Liangyu Cui, Zhangwen Deng, Yue Ma, Diancheng Yang, Yanan Gong, Yanchun Xu, Shuhui Yang, Song Huang 2023. Protocols for the assembly and annotation of snake genomes. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8j6e9g2w/v2Version created by Boyang Liu
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 06, 2023
Last Modified: February 09, 2023
Collection Integer ID: 76433
Keywords: Bungarus multicinctus, genome assembly, procotols' collection
Abstract
Background: Snakes are one of the most important wildlife resources and are widely distributed. Bungarus multicinctus is a highly venomous snake and is distributed in central and southern China. In recent years, venomous snake bites have gradually increased. Genomic resources are significant for understanding the evolution of a specie and the molecular mechanisms of toxin production.
Find: Here we present a highly- continuous genome assembly of B. multicinctus. The genome size of B. multicinctus is 1.51Gb. The maximal length of scaffold is 39.68M and the N50 length is 6.55M. The B. multicinctus has a GC content of 37.8%.

Files
Protocol
Icon representing the file DNA extraction protocol for snake genomic sequencing by using AxyPrepTM Multisource Genomic DNA Miniprep Kit
Name
DNA extraction protocol for snake genomic sequencing by using AxyPrepTM Multisource Genomic DNA Miniprep Kit
Version 2
,
Boyang Liu
Protocol
Icon representing the file RNA extraction protocol for snake genomes using TRlZOL reagent(Invitrogen)
Name
RNA extraction protocol for snake genomes using TRlZOL reagent(Invitrogen)
Version 2
,
Boyang Liu
Protocol
Icon representing the file BGISEQ-500 WGS library construction
Name
ForkBGISEQ-500 WGS library construction
Version 2
, GigaScience Press
Hongling ZhouGigaScience Press
Protocol
Icon representing the file BGISEQ-500 Sequencing
Name
ForkBGISEQ-500 Sequencing
Version 2
, GigaScience Press
Hongling ZhouGigaScience Press
Protocol
Icon representing the file STLFR library construction for snake genomes
Name
ForkSTLFR library construction for snake genomes
Version 1
,
Boyang Liu
Protocol
Icon representing the file The annotation pipeline for the genome of a snake
Name
ForkThe annotation pipeline for the genome of a snake
Version 1
,
Boyang Liu