Mix 100uL of dissociated cell suspension with 100uL of antibody cocktail containing 2x concentration of each of the following antibodies: anti-CD45_PerCP, anti-CD3_Pacific Blue, anti-CD8_BV605, anti-CD4_FITC, anti-CD11b_APC, OTS8-PE tetramer, and fixable viability dye eFluor 780.